6H2E
Structure of the soluble AhlC of the tripartite alpha-pore forming toxin, AHL, from Aeromonas hydrophila.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-27 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9796 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 134.710, 134.710, 145.280 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 58.330 - 2.350 |
| R-factor | 0.22874 |
| Rwork | 0.227 |
| R-free | 0.27364 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.534 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | SHELXDE |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.330 | 2.410 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rpim | 0.023 | 0.639 |
| Number of reflections | 32942 | 97395 |
| <I/σ(I)> | 19.8 | 1.4 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 39.3 | 40.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 290 | 0.2 M Magnesium chloride 0.1 M Tris pH 7 10 % (w/v) PEG8000 |
