6H1D

Crystal structure of C21orf127-TRMT112 in complex with SAH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-08-09
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.2810
Spacegroup name	P 42 21 2
Unit cell lengths	71.841, 71.841, 131.113
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	65.560 - 1.940
R-factor	0.1891
Rwork	0.187
R-free	0.23740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3q87 4qtt
RMSD bond length	0.007
RMSD bond angle	0.914
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	65.580	2.010
High resolution limit [Å]	1.940	1.940
Rmerge	0.178	0.903
Rpim	0.036	0.186
Number of reflections	26240
<I/σ(I)>	16.42	3.49
Completeness [%]	100.0	99.88
Redundancy	24.8	24.2
CC(1/2)	0.999	0.643

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M HEPES pH 7.5, 1.1-1.3 M sodium citrate