6H16

Structure of LRP6 P3E3P4E4 in complex with VHH L-P2-D07

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-21
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.8731
Spacegroup name	P 21 21 21
Unit cell lengths	92.795, 105.954, 164.191
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	82.096 - 2.900
R-factor	0.2127
Rwork	0.211
R-free	0.25180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4a0p
Data reduction software	DIALS
Data scaling software	Aimless (0.5.32)
Phasing software	PHASER
Refinement software	PHENIX (1.13rc1_2958)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	164.190	164.190	3.030
High resolution limit [Å]	2.900	10.050	2.900
Rmerge	0.107	0.043	1.029
Rmeas	0.117	0.048	1.117
Rpim	0.046	0.022	0.431
Total number of observations	233566
Number of reflections	36674	1008	4407
<I/σ(I)>	8.8
Completeness [%]	100.0	100	100
Redundancy	6.4	5.6	6.6
CC(1/2)	0.986	0.978	0.452

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	0.1 M MES pH 5.0, 10 % w/v PEG 6000