6H15

Structure of LRP6 P3E3P4E4 in complex with VHH L-P2-B10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-03
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.8731
Spacegroup name	P 65
Unit cell lengths	118.314, 118.314, 249.951
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	94.806 - 2.600
R-factor	0.2008
Rwork	0.198
R-free	0.24690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4a0p
Data reduction software	DIALS
Data scaling software	Aimless (0.5.32)
Phasing software	PHASER
Refinement software	PHENIX (1.13rc1_2958)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	249.950	249.950	2.670
High resolution limit [Å]	2.600	11.630	2.600
Rmerge	0.219	0.106	1.004
Rmeas	0.232	0.112	1.100
Rpim	0.074	0.035	0.433
Total number of observations	556037	6940	24042
Number of reflections	60113	717	3987
<I/σ(I)>	6.8	14	1.4
Completeness [%]	99.0	100	89.6
Redundancy	9.2	9.7	6
CC(1/2)	0.989	0.995	0.474

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	0.1 M sodium citrate, 0.2 M sodium acetate trihydrate pH 5.5, 10 % PEG w/v 4000