6H06
FAB CBTAU-22.1 IN COMPLEX WITH TAU PEPTIDE V1088-5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-01-09 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 108.055, 96.209, 108.984 |
| Unit cell angles | 90.00, 112.81, 90.00 |
Refinement procedure
| Resolution | 100.460 - 2.630 |
| R-factor | 0.21701 |
| Rwork | 0.216 |
| R-free | 0.26963 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.237 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.460 | 2.880 |
| High resolution limit [Å] | 2.630 | 2.630 |
| Rmerge | 0.084 | 0.435 |
| Number of reflections | 59335 | |
| <I/σ(I)> | 9.97 | 2.7 |
| Completeness [%] | 96.5 | 97.4 |
| Redundancy | 2.8 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.20 M KCl, 0.10 M Hepes/NaOH pH=7, 21.2 % (w/v) PEG 5K MME |
