6H02

Crystal structure of human Mediator subunit MED23

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-09-15
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.98011
Spacegroup name	P 1 21 1
Unit cell lengths	75.650, 137.180, 90.110
Unit cell angles	90.00, 110.98, 90.00

Refinement procedure

Resolution	15.000 - 2.800
R-factor	0.201
Rwork	0.199
R-free	0.23990
Structure solution method	MIRAS
RMSD bond length	0.002
RMSD bond angle	0.469
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHARP
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.900
High resolution limit [Å]	2.800	2.800
Rmeas	0.143	1.470
Number of reflections	42643	4250
<I/σ(I)>	14.2
Completeness [%]	99.8	99.9
Redundancy	20	20
CC(1/2)	0.999	0.887

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	294	10% PEG 6000 100mM HEPES