6GZW
Ferric DtpA from Streptomyces lividans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-3 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-15 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9677 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.118, 71.032, 78.089 |
| Unit cell angles | 90.00, 92.83, 90.00 |
Refinement procedure
| Resolution | 77.990 - 1.410 |
| R-factor | 0.1905 |
| Rwork | 0.189 |
| R-free | 0.21630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5map |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.513 |
| Data reduction software | EDNA |
| Data scaling software | Aimless (0.5.29) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 77.990 | 1.460 |
| High resolution limit [Å] | 1.410 | 1.410 |
| Rmerge | 0.127 | 1.253 |
| Rmeas | 0.141 | 1.391 |
| Rpim | 0.060 | 0.593 |
| Number of reflections | 118339 | 9905 |
| <I/σ(I)> | 7.8 | |
| Completeness [%] | 93.9 | 80.4 |
| Redundancy | 5.2 | 5.1 |
| CC(1/2) | 0.997 | 0.529 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | Crystals were grown by the vapour diffusion hanging drop method using a ferric protein solution at 13 mg/ml equilibrated against a reservoir consisting of 17-24% PEG 3000 and 50-100 mM sodium citrate, pH 5.5. |
