6GZL

Complex between the dynein light chain DYNLL1/DLC8 and a peptide from the large myelin-associated glycoprotein L-MAG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-02-04
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.91841
Spacegroup name	P 61 2 2
Unit cell lengths	43.730, 43.730, 205.390
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.871 - 1.953
R-factor	0.214
Rwork	0.212
R-free	0.25110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3zke
RMSD bond length	0.008
RMSD bond angle	0.945
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.000
High resolution limit [Å]	1.950	1.950
Rmerge	0.213	1.812
Rmeas	0.222	1.901
Number of reflections	6139	26
<I/σ(I)>	13.34	1.3
Completeness [%]	65.7	3.9
Redundancy	13	11.4
CC(1/2)	0.998	0.469

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	0.1 M Bis-Tris pH 6.5, 0.2 M lithium sulfate, and 25% (w/v) PEG 3350