6GZJ

Complex between the dynein light chain DYNLL1/DLC8 and the specific domain of large myelin-associated glycoprotein L-MAG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-12-11
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.07175
Spacegroup name	P 61 2 2
Unit cell lengths	44.050, 44.050, 204.460
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.148 - 1.977
R-factor	0.1931
Rwork	0.192
R-free	0.22890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3zke
RMSD bond length	0.013
RMSD bond angle	1.135
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2152: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.030
High resolution limit [Å]	1.977	1.977
Rmerge	0.107	0.813
Rmeas	0.110	0.854
Number of reflections	7535	62
<I/σ(I)>	17	3.9
Completeness [%]	82.8	9.7
Redundancy	17.6	10.9
CC(1/2)	0.999	0.902

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	0.1 M Bis-Tris pH 6.5, 0.2 M lithium sulfate, and 25% (w/v) PEG 3350