6GZA

Structure of murine leukemia virus capsid C-terminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2015-08-14
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	P 31 2 1
Unit cell lengths	71.198, 71.198, 77.068
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.599 - 1.890
R-factor	0.1783
Rwork	0.176
R-free	0.21820
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.724
Data reduction software	XDS
Data scaling software	pointless
Phasing software	SHELXDE
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.600	1.990
High resolution limit [Å]	1.890	1.890
Number of reflections	39681
<I/σ(I)>	18.9
Completeness [%]	100.0
Redundancy	10.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	37 mg/ml protein, 4% PEG 3350, 0.1 M HEPES pH 8.2, 5 mM CoCl2, 5 mM CdCl2, 5 mM MgCl2 and 5 mM NiCl2