6GYX
Crystal structure of DacA from Staphylococcus aureus in complex with ApCpp
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-11 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.092, 80.424, 96.195 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.279 - 2.600 |
| R-factor | 0.2328 |
| Rwork | 0.231 |
| R-free | 0.25760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6gyw |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.700 | 2.720 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.116 | 2.115 |
| Number of reflections | 10930 | 1291 |
| <I/σ(I)> | 10.2 | 2 |
| Completeness [%] | 99.3 | 99.2 |
| Redundancy | 12.2 | 12.3 |
| CC(1/2) | 0.997 | 0.825 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 10 mM MgCl2, 50 mM MES pH 5.8, 0.2M KCl, 3% Peg8000 |
