Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-03-29 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.6 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.630, 85.710, 108.530 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.824 - 2.500 |
| R-factor | 0.200698514255 |
| Rwork | 0.198 |
| R-free | 0.24490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4p9r |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.521 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELX |
| Refinement software | PHENIX (1.11rc1_2513) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.830 | 2.589 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 34663 | |
| <I/σ(I)> | 3.16 | |
| Completeness [%] | 96.5 | |
| Redundancy | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 Sodium chloride, 0.1 M BIS-TRIS pH 6.5, 25% w/v PEG 3,350 or (ii) 0.2 Sodium chloride, 0.1 M HEPES pH 7.5, 25% w/v PEG 3,350 |
