6GYF

Crystal structure of NadR protein in complex with NR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-12
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9762
Spacegroup name	P 64 2 2
Unit cell lengths	165.090, 165.090, 193.050
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.827 - 2.700
R-factor	0.1996
Rwork	0.198
R-free	0.23880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6gye
RMSD bond length	0.009
RMSD bond angle	1.054
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((dev_3150))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.827	2.800
High resolution limit [Å]	2.700	2.700
Number of reflections	41587
<I/σ(I)>	10
Completeness [%]	96.0
Redundancy	11

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	1M (NH4)2SO4, 100mM Na Citrate pH 6.5, 10mM MgCl2 and 10% glucose