6GY4

Crystal structure of the N-terminal KH domain of human BICC1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-09-30
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9212
Spacegroup name	C 2 2 21
Unit cell lengths	44.406, 153.164, 86.235
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.740 - 1.986
R-factor	0.2579
Rwork	0.256
R-free	0.30350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1zzk
RMSD bond length	0.003
RMSD bond angle	0.514
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.740	2.164
High resolution limit [Å]	1.986	1.986
Rmerge	0.099	0.949
Rpim	0.043	0.457
Number of reflections	11724	576
<I/σ(I)>	10	1.6
Completeness [%]	90.5	67.5
Redundancy	6.4	5.2
CC(1/2)	0.999	0.682

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.1 M Lithium Sulfate, 50 % PEG 400, 0.1 M acetate pH 4.0