6GY3

Crystal Structure of C. glutamicum AmtR bound to glnA operator DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-11-09
Detector	RAYONIX MX-225
Wavelength(s)	0.91841
Spacegroup name	P 21 21 21
Unit cell lengths	63.320, 76.150, 117.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.290 - 2.680
R-factor	0.21
Rwork	0.208
R-free	0.25100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5mqq
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.289	2.750
High resolution limit [Å]	2.680	2.680
Rmerge	0.072	0.618
Number of reflections	16335
<I/σ(I)>	21.46	3.39
Completeness [%]	98.8	90.1
Redundancy	6.964	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	0.2 M ammonium acetate, 0.1 M Tris-HCl, pH 8.5, 25% PEG 3350