6GXJ
X-ray structure of DiRu-1-encapsulated Apoferritin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-30 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979482 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 180.404, 180.404, 180.404 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.260 - 1.430 |
| R-factor | 0.174 |
| Rwork | 0.174 |
| R-free | 0.19100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5erk |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.860 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | BUSTER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 104.160 | 1.466 |
| High resolution limit [Å] | 1.430 | 1.430 |
| Rpim | 0.036 | 0.739 |
| Number of reflections | 45764 | 2274 |
| <I/σ(I)> | 13.7 | |
| Completeness [%] | 97.7 | 69.1 |
| Redundancy | 37.3 | |
| CC(1/2) | 0.996 | 0.739 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 0.6-0.8 M (NH4)2SO4, 0.1 M Tris HCl pH 7.7, 50 mM CDSO4 |
