6GX6

Crystal structure of IMP3 RRM12 in complex with RNA (ACAC)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-11
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.9999
Spacegroup name	P 31 2 1
Unit cell lengths	75.440, 75.440, 66.260
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.520 - 2.000
R-factor	0.1834
Rwork	0.180
R-free	0.23590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6fq1
RMSD bond length	0.007
RMSD bond angle	1.017
Data reduction software	MOSFLM (CCP4 7.0.050)
Data scaling software	SCALA (CCP4 7.0.050)
Phasing software	PHASER (CCP4 7.0.050)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	66.260	2.110
High resolution limit [Å]	2.000	2.000
Rmerge	0.092	1.165
Number of reflections	15116	2153
<I/σ(I)>	18.2	2.4
Completeness [%]	100.0	100
Redundancy	12.6	12
CC(1/2)	0.999	0.600

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293.15	0.2M KH2PO4, 20% PEG3350