6GX0
Blood group synthase AAGlyB in complex with UDP-Gal and cryoprotected with PEG 3350
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-08-28 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.072 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 52.849, 148.920, 80.039 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.250 |
R-factor | 0.12333 |
Rwork | 0.122 |
R-free | 0.15081 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 3zgg |
RMSD bond length | 0.011 |
RMSD bond angle | 1.666 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.290 | 1.270 |
High resolution limit [Å] | 1.250 | 1.250 |
Number of reflections | 87298 | |
<I/σ(I)> | 11 | |
Completeness [%] | 99.9 | |
Redundancy | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | MOPS buffer, Magnesium chloride, ammonium sulfate and PEG 3350 |