6GWJ

Human OSGEP / LAGE3 / GON7 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-01-25
Detector	PSI PILATUS 6M
Wavelength(s)	0.978570
Spacegroup name	P 43
Unit cell lengths	105.120, 105.120, 51.350
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.140 - 1.950
R-factor	0.188
Rwork	0.186
R-free	0.21700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.010
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.140	46.140	2.060
High resolution limit [Å]	1.950	5.800	1.950
Rmerge	0.066	0.033	1.097
Rmeas	0.071	0.035	1.188
Number of reflections	41359	1639	6542
<I/σ(I)>	15.97	49.81	1.38
Completeness [%]	99.6	99.5	98.1
Redundancy	7.308	6.969	6.862
CC(1/2)	0.999	0.999	0.585

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	of PEG 4000, 0.1 M Tris HCl pH 8.5 and 0.2 M Magnesium Chloride