6GWH
Outward-facing conformation of a multidrug resistance MATE family transporter of the MOP superfamily.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-10-08 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 95.830, 95.830, 369.110 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.970 - 2.800 |
| R-factor | 0.2589 |
| Rwork | 0.258 |
| R-free | 0.27960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4mlb |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.402 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.970 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.973 | |
| Number of reflections | 16175 | 4495 |
| <I/σ(I)> | 7.23 | |
| Completeness [%] | 97.3 | |
| Redundancy | 4.2 | |
| CC(1/2) | 0.995 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 5 | 295 | 30% PEG 500, 100 mM Na3C6H5O7-C6H8O7, 100 mM NaCl |
