6GVV
Mutant M16A of RNA dependent RNA polymerase 3D from Foot-and-Mouth disease Virus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97166 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 93.120, 93.120, 121.050 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.600 - 2.350 |
| R-factor | 0.22171 |
| Rwork | 0.220 |
| R-free | 0.26324 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1u09 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.884 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | REFMAC (rigid body refinement using as a model 1U09) |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.480 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.084 | 0.426 |
| Rpim | 0.035 | |
| Number of reflections | 22406 | |
| <I/σ(I)> | 11.4 | |
| Completeness [%] | 98.1 | |
| Redundancy | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 30% PEG 4000, 0.2M magnesium acetate, 0.1M MES[2-(N-morpholino) ethanesulfonic acid] pH 6.0 and 4% a-butyrolactone. |
