6GVP
TAILSPIKE PROTEIN MUTANT E372Q (DELTA N471/S472) OF E. COLI BACTERIOPHAGE HK620 IN COMPLEX WITH HEXASACCHARIDE
Replaces: 4XQHExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-11-06 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.91841 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 74.125, 74.125, 175.122 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.780 - 1.710 |
| R-factor | 0.1382 |
| Rwork | 0.136 |
| R-free | 0.17410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xor |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.825 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.780 | 1.740 |
| High resolution limit [Å] | 1.710 | 1.710 |
| Rmeas | 0.088 | 0.786 |
| Number of reflections | 61232 | 3127 |
| <I/σ(I)> | 19.8 | 2.8 |
| Completeness [%] | 99.8 | 97.3 |
| Redundancy | 6.1 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.1 M TRIS-HCL, 3.5 M SODIUM FORMATE PH 8.5 |
