6GVC
Structure of ArhGAP12 bound to G-Actin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-05-07 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 101.650, 130.240, 109.300 |
| Unit cell angles | 90.00, 111.17, 90.00 |
Refinement procedure
| Resolution | 54.132 - 2.600 |
| R-factor | 0.209 |
| Rwork | 0.207 |
| R-free | 0.25080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v52 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.615 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.132 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.170 | 0.770 |
| Rmeas | 0.180 | 0.910 |
| Rpim | 0.060 | 0.480 |
| Number of reflections | 81113 | 7746 |
| <I/σ(I)> | 10.8 | 1.66 |
| Completeness [%] | 99.4 | 95.83 |
| Redundancy | 6.2 | 3.4 |
| CC(1/2) | 0.990 | 0.620 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% PEG 3350, 0.2M Sodium Thiocyanate 0.1 M Bis Tris Propane pH6.5 |
