6GVC

Structure of ArhGAP12 bound to G-Actin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-05-07
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.97
Spacegroup name	P 1 21 1
Unit cell lengths	101.650, 130.240, 109.300
Unit cell angles	90.00, 111.17, 90.00

Refinement procedure

Resolution	54.132 - 2.600
R-factor	0.209
Rwork	0.207
R-free	0.25080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2v52
RMSD bond length	0.004
RMSD bond angle	0.615
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.132	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.170	0.770
Rmeas	0.180	0.910
Rpim	0.060	0.480
Number of reflections	81113	7746
<I/σ(I)>	10.8	1.66
Completeness [%]	99.4	95.83
Redundancy	6.2	3.4
CC(1/2)	0.990	0.620

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20% PEG 3350, 0.2M Sodium Thiocyanate 0.1 M Bis Tris Propane pH6.5