6GUV

BTB domain of mouse PATZ1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-18
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97625
Spacegroup name	P 41 21 2
Unit cell lengths	43.232, 43.232, 162.946
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.786 - 2.290
R-factor	0.2112
Rwork	0.209
R-free	0.24480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1buo
RMSD bond length	0.002
RMSD bond angle	0.433
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.230	2.370
High resolution limit [Å]	2.290	2.290
Rmerge	0.050	0.670
Rmeas	0.076	0.950
Rpim	0.054	0.670
Number of reflections	7573	724
<I/σ(I)>	9.91	1.39
Completeness [%]	99.6	99.18
Redundancy	2	2
CC(1/2)	0.998	0.551

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	291	MMT ( DL-Malic acid, MES monohydrate ,Tris) 0.1M PEG 1500, 25% w/v