6GUU
Structure of CHD5 PHD2 - tandem chromodomains
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-11-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97265 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 131.628, 131.628, 84.569 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.940 - 2.950 |
| R-factor | 0.2091 |
| Rwork | 0.206 |
| R-free | 0.26300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4o9i |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.686 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.940 | 3.056 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.025 | 0.541 |
| Rmeas | 0.035 | 0.765 |
| Number of reflections | 16801 | |
| <I/σ(I)> | 17.18 | |
| Completeness [%] | 100.0 | |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.2 M ammonium nitrate 20 % PEG 3350 2 % benzamidine hydrochloride |
