6GUM

Structure of the A.thaliana E1 UFD domain in complex with E2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-12
Detector	DECTRIS PILATUS 300K
Wavelength(s)	0.97923
Spacegroup name	P 1 21 1
Unit cell lengths	36.158, 58.817, 70.230
Unit cell angles	90.00, 92.05, 90.00

Refinement procedure

Resolution	45.080 - 1.792
R-factor	0.1803
Rwork	0.178
R-free	0.22030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1a3s
RMSD bond length	0.017
RMSD bond angle	1.623
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.810	1.798
High resolution limit [Å]	1.792	1.792
Rmerge	0.094
Rmeas	0.108
Rpim	0.061
Number of reflections	26999
<I/σ(I)>	8.5
Completeness [%]	97.2
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	15% PEG6000, 5% Glycerol