6GU4
CDK1/CyclinB/Cks2 in complex with CGP74514A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-05-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.513, 68.205, 166.086 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 83.180 - 2.730 |
| R-factor | 0.19947 |
| Rwork | 0.197 |
| R-free | 0.25272 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4y72 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.522 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 83.180 | 2.860 |
| High resolution limit [Å] | 2.730 | 2.730 |
| Rmerge | 0.270 | |
| Number of reflections | 20165 | |
| <I/σ(I)> | 5.2 | 1.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.7 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.1M MES/IMIDAZOLE BUFFER (PH6.7), 6.5% MPD, 5% PEG4K, 10% PEG1K PROTEIN AT 10-12 MG/ML + 0.5mM Inhibitor |
