6GU2
CDK1/CyclinB/Cks2 in complex with Flavopiridol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 78.132, 53.782, 180.213 |
| Unit cell angles | 90.00, 98.26, 90.00 |
Refinement procedure
| Resolution | 89.170 - 2.000 |
| R-factor | 0.19196 |
| Rwork | 0.189 |
| R-free | 0.23918 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4y72 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.799 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 89.170 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.090 | 1.010 |
| Number of reflections | 50412 | |
| <I/σ(I)> | 6.9 | 1.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.1M MES/IMIDAZOLE BUFFER (PH6.7), 6.5% MPD, 5% PEG4K, 10% PEG1K PROTEIN AT 10-12 MG/ML + 0.5mM Inhibitor |
