6GTT
Human STK10 bound to BIRB-796
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-20 |
| Detector | DECTRIS PILATUS3 R CdTe 300K |
| Wavelength(s) | 0.9282 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 100.667, 100.667, 145.786 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 87.180 - 2.250 |
| R-factor | 0.24358 |
| Rwork | 0.240 |
| R-free | 0.30104 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4use |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.111 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 87.180 | 2.320 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.070 | |
| Number of reflections | 21422 | 1920 |
| <I/σ(I)> | 22.8 | 2.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 17.7 | 18.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 277 | 20% PEG6000, 10% ethylene glycol, 0.1M MES pH 6.0, 0.1M magnesium chloride |
