6GT5

METTL16 MTase domain (crystal form 2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-02
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.8731
Spacegroup name	P 41 21 2
Unit cell lengths	89.550, 89.550, 179.130
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	80.100 - 2.450
R-factor	0.185
Rwork	0.182
R-free	0.24000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6gfn
RMSD bond length	0.009
RMSD bond angle	1.100
Data reduction software	XDS (BUILT 20171111)
Data scaling software	Aimless (0.6.2)
Phasing software	PHASER (2.7.17)
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	80.100	2.800
High resolution limit [Å]	2.450	2.450
Rmerge	0.360	1.580
Rmeas	0.331	1.660
Rpim	0.124	0.500
Number of reflections	12391	619
<I/σ(I)>	4.5	1.6
Completeness [%]	93.3	79.9
Redundancy	6.9	10.8
CC(1/2)	0.967	0.650

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.1M Bis-Tris propane, 0.2 M pottassium-sodium tartrate, 20% PEG 3350