6GSP

RIBONUCLEASE T1/3'-GMP, 15 WEEKS

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1997-11
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	48.350, 51.110, 40.540
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.200
R-factor	0.195
Rwork	0.195
R-free	0.21700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rga
RMSD bond length	0.080
RMSD bond angle	25.600 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.851)
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.082 *	0.224 *
Total number of observations	11191 *
Number of reflections	5517
<I/σ(I)>	13.6	5.7
Completeness [%]	96.8	94.7
Redundancy	4.7	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.2		20 MM NAAC PH 4.2 2 MM CACL2 55 % MPD

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	RNase T1	15 (mg/ml)
2	1	drop	exo-cGPS	15 (mM)
3	1	drop		20 (mM)
4	1	drop		2 (mM)
5	1	reservoir	MPD	55 (%(v/v))