6GSK

Structure of T. thermophilus 70S ribosome complex with mRNA, tRNAfMet and near-cognate tRNAThr in the A-site

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-27
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.2399
Spacegroup name	P 21 21 21
Unit cell lengths	209.820, 449.750, 618.710
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	224.875 - 3.360
R-factor	0.2004
Rwork	0.200
R-free	0.26420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4v8d
RMSD bond length	0.009
RMSD bond angle	1.490
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	224.875	224.875	3.450
High resolution limit [Å]	3.360	15.030	3.360
Rmerge	0.359	0.042	3.744
Rmeas	0.364	0.042	3.826
Number of reflections	1610361	17962	119307
<I/σ(I)>	11.53	82.05	0.99
Completeness [%]	99.9	99.9	100
Redundancy	36.023	39.593	23.564
CC(1/2)	0.998	0.998	0.316

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	Tris-OAc, KSCN, PEG-20000, PEG-550mme