6GSJ

Structure of T. thermophilus 70S ribosome complex with mRNA, tRNAfMet and cognate tRNAThr in the A-site

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-09-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.2399
Spacegroup name	P 21 21 21
Unit cell lengths	209.410, 449.240, 618.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	224.620 - 2.960
R-factor	0.1961
Rwork	0.196
R-free	0.25190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4v6g
RMSD bond length	0.009
RMSD bond angle	1.536
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	224.620	224.620	3.040
High resolution limit [Å]	2.960	13.240	2.960
Rmerge	0.290	0.041	4.647
Rmeas		0.042	4.705
Total number of observations	204674388
Number of reflections	2349022	26255	174084
<I/σ(I)>	29.2	127.93	1.03
Completeness [%]	100.0	100	100
Redundancy	87.132	95.881	41.677
CC(1/2)	0.999	0.999	0.248

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Tris-OAc, KSCN, PEG-20000, PEG-550mme