6GS7

Crystal structure of peptide transporter DtpA-nanobody in glycine buffer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.976
Spacegroup name	P 21 21 21
Unit cell lengths	55.145, 120.534, 163.433
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.939 - 3.300
R-factor	0.212
Rwork	0.210
R-free	0.24580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6gs1
RMSD bond length	0.002
RMSD bond angle	0.482
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.940	3.417
High resolution limit [Å]	3.300	3.300
Rmerge	0.247
Number of reflections	16935
<I/σ(I)>	7.04
Completeness [%]	99.0
Redundancy	8.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	PEG 400, calcium chloride, glycine buffer