6GRS
Paired immunoglobulin-like receptor B (PirB) or Leukocyte immunoglobulin-like receptor subfamily B member 3 (LILRB3) full extracellular domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-08-29 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.690, 185.289, 99.080 |
| Unit cell angles | 90.00, 105.56, 90.00 |
Refinement procedure
| Resolution | 52.686 - 3.400 |
| R-factor | 0.2581 |
| Rwork | 0.256 |
| R-free | 0.30750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2d3v 4lla |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.604 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.690 | 3.630 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.108 | 0.824 |
| Number of reflections | 25778 | 4683 |
| <I/σ(I)> | 6.9 | 1.8 |
| Completeness [%] | 98.8 | 99.2 |
| Redundancy | 3.2 | 3.2 |
| CC(1/2) | 0.949 | 0.586 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293.15 | 1.2 M LiCl, 12% PEG 6000m 0.1 M Citric acid buffer pH 5.5 |
