6GRJ
Structure of the AhlB pore of the tripartite alpha-pore forming toxin, AHL, from Aeromonas hydrophila.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-04 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97938 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 363.644, 116.526, 217.409 |
| Unit cell angles | 90.00, 118.01, 90.00 |
Refinement procedure
| Resolution | 102.670 - 2.940 |
| R-factor | 0.22319 |
| Rwork | 0.222 |
| R-free | 0.23865 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.570 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | SHELXDE |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 107.580 | 2.990 |
| High resolution limit [Å] | 2.940 | 2.940 |
| Number of reflections | 169188 | |
| <I/σ(I)> | 1.127 | |
| Completeness [%] | 99.0 | |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289.15 | 60% Methyl-2,4-Pentanediol (MPD), 0.2M Ammonium phosphate, 0.1M Tris pH8.5 |
