6GRF
Crystal structure of the tandem DUF26 ectodomain from the Arabidopsis thaliana cysteine-rich receptor-like protein PDLP8.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-19 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 0.999871 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 143.862, 143.862, 59.722 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.100 - 1.950 |
| R-factor | 0.23018 |
| Rwork | 0.229 |
| R-free | 0.26189 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6gre |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.148 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.100 | 2.070 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmeas | 0.320 | 3.620 |
| Number of reflections | 51859 | 8245 |
| <I/σ(I)> | 8.82 | 1 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 20.3 | 19.7 |
| CC(1/2) | 0.998 | 0.647 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 17.5 % polyethylene glycol 4,000, 0.1 M citrate pH 5.5, 20 % (v/v) 2-propanol |
