6GRE
Crystal structure of the tandem DUF26 ectodomain from the Arabidopsis thaliana cysteine-rich receptor-like protein PDLP5.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-08 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.000033 |
| Spacegroup name | P 1 |
| Unit cell lengths | 41.760, 47.970, 62.190 |
| Unit cell angles | 97.70, 102.72, 99.86 |
Refinement procedure
| Resolution | 37.770 - 1.290 |
| R-factor | 0.18762 |
| Rwork | 0.186 |
| R-free | 0.20988 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.502 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.770 | 1.320 |
| High resolution limit [Å] | 1.290 | 1.290 |
| Rmeas | 0.049 | 1.290 |
| Number of reflections | 108820 | 7623 |
| <I/σ(I)> | 12.56 | 1.13 |
| Completeness [%] | 94.5 | 89.4 |
| Redundancy | 3.6 | 3.7 |
| CC(1/2) | 0.999 | 0.557 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 298 | 17.5 % polyethylene glycol 4,000, 250 mM (NH4)2SO4 |
