6GRD
eukaryotic junction-resolving enzyme GEN-1 binding with Cesium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-02 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.2 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 97.390, 97.390, 119.000 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 68.812 - 2.660 |
| R-factor | 0.2454 |
| Rwork | 0.244 |
| R-free | 0.26240 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.307 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASES |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.812 | 2.730 |
| High resolution limit [Å] | 2.660 | 2.660 |
| Number of reflections | 19265 | |
| <I/σ(I)> | 15 | |
| Completeness [%] | 99.9 | |
| Redundancy | 19.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 279 | PEG10000, NaCl, magnesium chloride, cesium chloride |
