6GQD

Structure of human galactose-1-phosphate uridylyltransferase (GALT), with crystallization epitope mutations A21Y:A22T:T23P:R25L

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-21
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.92818
Spacegroup name	P 21 21 2
Unit cell lengths	72.647, 96.748, 55.426
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	58.093 - 1.523
R-factor	0.1636
Rwork	0.162
R-free	0.18770
Data reduction software	xia2
Data scaling software	Aimless (0.5.26)
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	72.650	72.650	1.610
High resolution limit [Å]	1.520	4.820	1.520
Rmerge	0.087	0.032	0.836
Rmeas	0.095	0.036	0.905
Rpim	0.037	0.014	0.344
Total number of observations		12415	59094
Number of reflections	60414	2099	8705
<I/σ(I)>	12.6	40.3	2.3
Completeness [%]	99.9	99.5	100
Redundancy	6.5	5.9	6.8
CC(1/2)	0.999	0.999	0.888

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277.15	0.2M ammonium sulphate, 30% PEG4000