6GPN

Crystal Structure of the CsiD Glutarate Hydroxylase in complex with N-Oxalylglycine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-03
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.000
Spacegroup name	P 4 21 2
Unit cell lengths	121.380, 121.380, 136.570
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.914 - 2.200
R-factor	0.1873
Rwork	0.187
R-free	0.21140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6gpe
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.310	1.860
High resolution limit [Å]	1.800	1.800
Number of reflections	91254
<I/σ(I)>	17.7
Completeness [%]	100.0
Redundancy	26.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	3.5 M Sodium formate, 0.1 M BIS-TRIS propane pH 7.0