6GPM
Crystal structure of domain 2 from TmArgBP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-02-02 |
Detector | RIGAKU SATURN 944 |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 25.619, 49.730, 27.782 |
Unit cell angles | 90.00, 100.19, 90.00 |
Refinement procedure
Resolution | 14.180 - 1.730 |
R-factor | 0.13137 |
Rwork | 0.129 |
R-free | 0.16847 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4prs |
RMSD bond length | 0.014 |
RMSD bond angle | 1.674 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 1.770 |
High resolution limit [Å] | 1.730 | 1.730 |
Rmerge | 0.040 | |
Number of reflections | 6898 | |
<I/σ(I)> | 30.2 | |
Completeness [%] | 95.1 | |
Redundancy | 1.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | Crystals of the D2 domain were obtained using a protein concentration of 14 mg/ml in a solution containing 0.1M Bis-Tris (pH 6.5) and 28% (w/v) polyethylene glycol 2,000. |