6GPK

Crystal structure of human GDP-D-mannose 4,6-dehydratase (E157Q) in complex with GDP-Man

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.92819
Spacegroup name	P 21 21 21
Unit cell lengths	93.730, 114.532, 140.061
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	88.660 - 1.470
R-factor	0.14226
Rwork	0.141
R-free	0.17434
RMSD bond length	0.013
RMSD bond angle	1.505
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	140.060	1.550
High resolution limit [Å]	1.470	1.470
Number of reflections	254599
<I/σ(I)>	17.2	2.5
Completeness [%]	100.0
Redundancy	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20% PEG3350, 0.15M DL- malic acid