6GP0

Structure of mEos4b in the red fluorescent state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-27
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	38.610, 57.900, 102.640
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.138 - 1.500
R-factor	0.1555
Rwork	0.154
R-free	0.19000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6goy
RMSD bond length	0.004
RMSD bond angle	0.866
Data reduction software	XDS (Version October 15, 2015)
Data scaling software	XSCALE (Version October 15, 2015)
Phasing software	PHENIX (1.11)
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	36.138	36.138	1.540
High resolution limit [Å]	1.500	6.700	1.500
Rmerge	0.047	0.014	0.542
Rmeas	0.059	0.017	0.691
Number of reflections	36532	421	2643
<I/σ(I)>	13.87	48.99	1.79
Completeness [%]	96.3	84	95.4
Redundancy	2.455	2.553	2.401
CC(1/2)	0.999	0.999	0.631

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	100 mM HEPES, 30 % PEG 1000