6GOY

Structure of mEos4b in the green fluorescent state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	38.600, 58.090, 103.270
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.593 - 1.650
R-factor	0.1693
Rwork	0.168
R-free	0.20390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1zux
RMSD bond length	0.004
RMSD bond angle	0.786
Data reduction software	XDS (VERSION Jun 1, 2017)
Data scaling software	XSCALE (VERSION Jun 1, 2017)
Phasing software	PHASER (2.7.16)
Refinement software	PHENIX (1.11)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	38.593	38.593	1.690
High resolution limit [Å]	1.650	7.380	1.650
Rmerge	0.100	0.066	0.507
Rmeas	0.121	0.080	0.625
Number of reflections	27933	328	2083
<I/σ(I)>	6.59	13.8	1.82
Completeness [%]	97.2	85.9	99
Redundancy	2.753	2.762	2.741
CC(1/2)	0.991	0.987	0.699

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	100 mM HEPES, 30 % PEG 1000