6GOS
E. coli Microcin synthetase McbBCD complex with pro-MccB17 bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-02-01 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9790 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 180.960, 83.400, 86.900 |
Unit cell angles | 90.00, 91.45, 90.00 |
Refinement procedure
Resolution | 57.390 - 2.100 |
R-factor | 0.174 |
Rwork | 0.172 |
R-free | 0.21200 |
Structure solution method | SAD |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.17) |
Phasing software | CRANK2 |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 57.390 | 57.390 | 2.150 |
High resolution limit [Å] | 2.100 | 9.390 | 2.100 |
Rmerge | 0.153 | 0.048 | 1.489 |
Rmeas | 0.161 | 0.050 | 1.568 |
Rpim | 0.049 | 0.016 | 0.485 |
Total number of observations | 800263 | ||
Number of reflections | 75405 | 895 | 5495 |
<I/σ(I)> | 10.7 | ||
Completeness [%] | 99.8 | 99.5 | 98.4 |
Redundancy | 10.6 | 9.7 | 10.2 |
CC(1/2) | 0.998 | 0.998 | 0.540 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | NULL |