6GOD
KRAS full length wild-type GPPNHP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-10 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.966 |
| Spacegroup name | H 3 |
| Unit cell lengths | 78.760, 78.760, 77.180 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 51.110 - 1.710 |
| R-factor | 0.18452 |
| Rwork | 0.182 |
| R-free | 0.23102 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4dst |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.876 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.110 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.051 | 0.468 |
| Rmeas | 0.062 | 0.566 |
| Number of reflections | 18963 | |
| <I/σ(I)> | 11.1 | 2.3 |
| Completeness [%] | 98.0 | 99.6 |
| Redundancy | 3 | 3.2 |
| CC(1/2) | 0.560 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 0.1 M TrisCl, 0.2 M NaOAc and 30-35 % PEG 4000 |
