6GN6

Alpha-L-fucosidase isoenzyme 1 from Paenibacillus thiaminolyticus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-09-11
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9201
Spacegroup name	P 21 21 21
Unit cell lengths	107.772, 148.758, 194.895
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.930 - 2.200
R-factor	0.169
Rwork	0.167
R-free	0.21700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2wvs
RMSD bond length	0.016
RMSD bond angle	1.825
Data reduction software	XDS (November 3, 2014)
Data scaling software	XDS (November 3, 2014)
Phasing software	MOLREP (11.2.08)
Refinement software	REFMAC (5.8.0222)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.930	2.240
High resolution limit [Å]	2.200	2.200
Rmerge	0.099	0.709
Number of reflections	157780
<I/σ(I)>	12.3	2.1
Completeness [%]	99.4	99.2
Redundancy	5	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291.15	25% (w/v) PEG 3350, 0.2 M Ammonium acetate, 0.1 M BIS-TRIS buffer, Additive: 50 mM Maltose