6GME

Structure of H. sapiens SPT6 tandem SH2 domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-27
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.999
Spacegroup name	P 1 21 1
Unit cell lengths	51.020, 81.100, 51.370
Unit cell angles	90.00, 115.08, 90.00

Refinement procedure

Resolution	46.210 - 1.802
R-factor	0.1923
Rwork	0.191
R-free	0.22310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3psj
RMSD bond length	0.006
RMSD bond angle	0.749
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX ((1.13_2998: ???))
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.210	1.867
High resolution limit [Å]	1.802	1.802
Rmerge	0.103
Rmeas	0.113
Rpim	0.046
Number of reflections	33254	2484
<I/σ(I)>	11.88	0.9
Completeness [%]	94.9	71.59
Redundancy	6.1	4.1
CC(1/2)	0.997	0.395

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	100 mM Bis-Tris pH 5.5, 200 mM MgCl2, 21-25 % (v/v) PEG 3350