6GLX
Structure of galectin-10 in complex with the Fab fragment of a Charcot-Leyden crystal solubilizing antibody, 4E8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-27 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.980058 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.326, 150.449, 94.504 |
| Unit cell angles | 90.00, 96.31, 90.00 |
Refinement procedure
| Resolution | 46.965 - 3.396 |
| R-factor | 0.3071 |
| Rwork | 0.303 |
| R-free | 0.36000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lcl |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.660 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.600 |
| High resolution limit [Å] | 3.390 | 3.390 |
| Rmeas | 0.219 | 1.070 |
| Number of reflections | 15646 | 2489 |
| <I/σ(I)> | 5.09 | 1.05 |
| Completeness [%] | 98.4 | 98.1 |
| Redundancy | 3.2 | 3.3 |
| CC(1/2) | 0.987 | 0.365 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M ammonium nitrate 20% PEG3350 |
